The study utilized secondary ion mass spectrometry to analyze stable nitrogen and carbon isotope assimilation in Nitrospira inopinata cells following exposure to 15N-guanidine and 13C-bicarbonate. Cells were fixed on gold-sputtered filters and attached to silicon wafers for analysis using a NanoSIMS 50L instrument.
New Nature article shows growth of comammox nitrifiers on guanidine
Fig. 1: Structure of the guanidinase enzyme of the comammox species Nitrospira inopinata, which converts guanidine into urea. The presumed entrance to a tunnel leading to the active site is highlighted in the left image. In the right section, the tunnel is shown as a green line and guanidine as a stick model. The structure was elucidated by the team led by Kristina Djinović-Carugo. C: Kristina Djinović-Carugo/University of Vienna
